PMO & PPMO
As a type of oligo molecule, Phosphorodiamidate morpholino oligomers (PMO) are short single-stranded DNA analogs that are built upon a backbone of morpholine rings connected by phosphorodiamidate linkages. They are routinely used for gene silencing and the recently developed PMO-based drug as excellent antisense reagents, however the synthesis of PMO remains challenging.
WuXi TIDES has developed high loading solid-phase PMO synthesis process that can achieve >50% yield, >75% crude purity, >90% final product purity with<0.045 EU/mg endotoxin.
Our chemistry knowhow enables various PMO 5' modifications with improved cleavage & de-protection process. Our optimized process offers freedom to operate without IP concerns.
We have customized PMO synthesis reactors up to 100L to support large-scale production. Our team successfully completed kilogram scale PMO development and manufacturing projects.
Our oligo and peptide API platforms are both in one site Changzhou, China. It's well positioned for oligo and peptide conjugate development and manufacturing, especially for Peptide-PMO (PPMO). To date, our team has completed dozens of PPMO projects. Below is a case study how different teams seamless collaborate with in-parallel activities, providing a fast, efficient and flexible solution for PPMO development.